Objectives : This study investigated the effects of various human saliva contamination and decontamination protocols on the shear bond strengths (SBS) of three adhesives on bovine superficial dentin.
Materials and methods : Adhesives tested were Adper™ Single Bond Plus Adhesive (SB+), Adper™ Scotchbond™ SE Self-Etch Adhesive (SBSE) and Adper™ Easy Bond Self-Etch Adhesive (EB) (all from 3M ESPE). A notched-edge shear method (Ultradent) was used to measure the SBS (composite: Filtek™ Z250 Universal Composite A2, 3M ESPE) with n = 10 except as noted in results table. Bonded specimens were tested after 24 h storage in water at 37 °C. Various application protocols were used (see table), with the following abbreviations:
-
A: adhesive application per manufacturer’s instructions for use (IFU)
-
C: light cure per IFU
-
S: fresh human saliva application, 15 s
-
D: air dry, 10 s
-
R: rinse with water, 10 s
-
A/Smix: mixture of 10% saliva/90% adhesive applied per IFU
Results : Mean (std dev) SBS in MPa and p -values (two-sample t -tests vs. control, p < 0.05 significance) are listed in the table.
| Set ID | Application protocol | SB+ | SBSE a | EB |
|---|---|---|---|---|
| 0 | A–C [control] | 36.1 (8.1) | 39.2 (10.5) | 39.6 (5.8) |
| 1 | S–A–C | 28.2 (6.0) p = 0.023 | 37.7 (12.1) p = 0.771 | 27.5 (10.6) p = 0.005 |
| 2 | A–C–S | 23.1 (10.5) p = 0.006 | 31.9 (7.7) p = 0.093 | 24.1 (6.0) p = 0.000 |
| 2A | A–C–S–R–D–A | 30.6 (6.7) p = 0.115 | 40.1 (9.7) p = 0.844 | 37.3 (7.9) p = 0.468 |
| 3 | A–S–C | 0.0 p = 0.000 | 2.9 (6.7) p = 0.000 | 0.0 p = 0.000 |
| 3A b | A–S–R–D–A–C | 16.9 (7.4) p = 0.000 | 26.1 (9.5) p = 0.009 | 34.0 (9.2) p = 0.121 |
| 4 c | A/Smix–C | 24.1 (8.9) ( n = 8) p = 0.009 | 16.6 (6.6) ( n = 9) p = 0.000 | 36.0 (8.1) ( n = 8) p = 0.268 |
Stay updated, free dental videos. Join our Telegram channel
VIDEdental - Online dental courses
