Objectives : To evaluate the morphology of the hybrid layer of one total etch and one self-etch DBA alter water storage and the effect of the application of 2% clorhexidine on its longevity.
Materials and methods : Twelve maxillary teeth (molars and premolars) had a class II cavity prepared with a 558 carbide bur leaving an enamel wall to separate a mesial cavity from a distal cavity. Teeth were separated in two groups ( n = 6), to receive either Adper Single Bond 2 (SB2) or Adper SE Plus (SEP) (3 M ESPE). In one cavity the DBA was applied following manufacturer’s instructions, on the other, 2% clorhexidine was applied for 30 s before the application of the DBA. After acid-etching with SB2 and before application of bottle A with SEP. Teeth were sectioned with a slow speed diamond disk (Isomet Slowspeed Saw) M-D and stored in water at 37 °C for 125 days. Then teeth were prepared for SEM observation and morphology of the hybrid layer was observed and measured under 3 parameters: clear image of hybrid layer; presence of resin tags in tubules; and length of resin tags. A grade of either 0 or 1 was given to each parameter (not present and present). Data was analyzed with a Kruskal–Wallis test calculated at a 0.05 significance level.
Results : Number of specimens graded “1” for each parameters in all study groups are listed in table.
| Treatment | Hybrid layer | Resin tags | Tags length |
|---|---|---|---|
| SB2 | |||
| w/o CHX | 3 | 3 | 1 |
| CHX | 6 | 6 | 2 |
| SEP | |||
| w/o CHX | 3 | 3 | 2 |
| CHX | 6 | 4 | 4 |
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