Saliva effect on dentin bond strength for three adhesives

Objectives : This study investigated the effects of various human saliva contamination and decontamination protocols on the shear bond strengths (SBS) of three adhesives on bovine superficial dentin.

Materials and methods : Adhesives tested were Adper™ Single Bond Plus Adhesive (SB+), Adper™ Scotchbond™ SE Self-Etch Adhesive (SBSE) and Adper™ Easy Bond Self-Etch Adhesive (EB) (all from 3M ESPE). A notched-edge shear method (Ultradent) was used to measure the SBS (composite: Filtek™ Z250 Universal Composite A2, 3M ESPE) with n = 10 except as noted in results table. Bonded specimens were tested after 24 h storage in water at 37 °C. Various application protocols were used (see table), with the following abbreviations:

  • A: adhesive application per manufacturer’s instructions for use (IFU)

  • C: light cure per IFU

  • S: fresh human saliva application, 15 s

  • D: air dry, 10 s

  • R: rinse with water, 10 s

  • A/Smix: mixture of 10% saliva/90% adhesive applied per IFU

Results : Mean (std dev) SBS in MPa and p -values (two-sample t -tests vs. control, p < 0.05 significance) are listed in the table.

Set ID Application protocol SB+ SBSE a EB
0 A–C [control] 36.1 (8.1) 39.2 (10.5) 39.6 (5.8)
1 S–A–C 28.2 (6.0) p = 0.023 37.7 (12.1) p = 0.771 27.5 (10.6) p = 0.005
2 A–C–S 23.1 (10.5) p = 0.006 31.9 (7.7) p = 0.093 24.1 (6.0) p = 0.000
2A A–C–S–R–D–A 30.6 (6.7) p = 0.115 40.1 (9.7) p = 0.844 37.3 (7.9) p = 0.468
3 A–S–C 0.0 p = 0.000 2.9 (6.7) p = 0.000 0.0 p = 0.000
3A b A–S–R–D–A–C 16.9 (7.4) p = 0.000 26.1 (9.5) p = 0.009 34.0 (9.2) p = 0.121
4 c A/Smix–C 24.1 (8.9) ( n = 8) p = 0.009 16.6 (6.6) ( n = 9) p = 0.000 36.0 (8.1) ( n = 8) p = 0.268
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Nov 30, 2017 | Posted by in Dental Materials | Comments Off on Saliva effect on dentin bond strength for three adhesives
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