The authors describe a simple and time saving technique for the ex-vivo production of oral mucosa keratinocytes by the direct explant method.
Primary cell culture of human oral tissue has many applications for maxillofacial surgery and oral biology research. There are two techniques in primary culture: the enzymatic and direct explant techniques. In the explant technique, after tissue extraction, the cells adhering and multiplying on the culture dish migrate out starting from the edges of the initial tissue affixed to the culture dish. After washing and disinfection, the tissue sample should be cut into small pieces, approximately 1–2 mm in size, and placed with the mucosal surface directly over the culture dish area. Considering the size of the specimen, the identification of the epithelial surface may be complex and time consuming. A simple technique to overcome this problem is described.
Prior to tissue sampling, the mucosal surface of the donor site was marked with surgical pen ( Figs. 1 and 2 ) which simplifies the identification of the epithelial surface during placement of the tissue sample on the culture dish area ( Fig. 3 ).