Modulation of VEGF-C expression by stress related mediator in oral squamous cell carcinoma cell lines

The aim of this study was to investigate the influence of norepinephrine on the expression of vascular endothelial growth factor type C (VEGF-C) in oral squamous carcinomas cell lines that contained beta-adrenergic receptors. Cell lines (SCC-9 and SCC-25) were stimulated with different concentrations of norepinephrine (0.1, 1 and 10 mM) and 1 μM of propranolol, and analyzed after 1, 6 and 24 h. Gene and protein expressions of VEGF-C were evaluated, respectively, by real time PCR and by ELISA. Data from experiments were subjected to analysis of variance, considering significant p ≤ 0.05. Dosage protein in supernatant cultures of malignant cells did not reflect the gene expression of VEGF-C. Only in the SCC-25 cell line was detected a significant inhibition of VEGF-C production by neoplastic cells, 24 h after stimulation with 10 μM norepinephrine ( p < 0.001). These results suggest that VEGF-C expression in oral squamous carcinomas cell lines seems not to be mediated by norepinephrine through the beta adrenergic receptor pathway.

Conflict of interest: None declared.

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Feb 5, 2018 | Posted by in Oral and Maxillofacial Surgery | Comments Off on Modulation of VEGF-C expression by stress related mediator in oral squamous cell carcinoma cell lines
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