Activated autologous bone marrow for bone tissue engineering in the maxillofacial area: clinical application in preprosthetic implant surgery

Stem cell therapy for tissue guided bone regeneration is a promising approach and widely studied in maxillofacial surgery i.e. bone regeneration and implant surgery.

Aim: The concept using mesenchymal stem cells (MSC) for bone regeneration has been considered here and applied with the aim to stimulate and accelerate bone regeneration is an atrophic mandible for further rehabilitation with implants.

Materials and methods: Bone marrow stimulation of iliac crest was performed 4 days before bone marrow stem cells harvesting using a solution of autologous platelet rich plasma activated by human recombinant soluble tissue factor (rhsTF) produced in GMP conditions. The surgery was performed on 4th day to increase the mandibular ridge and thickness using Bio-oss collagen as a scaffold. The Bio-Oss cubes were covered with collagen membrane screwed to the residual bone to secure stability. Thirty milliliters (3 × 10 ml) of bone marrow was taken from the stimulated site. Samples were centrifuged at 2000 rpm and the buffy coat of two syringes was injected in the scaffolds. A 3rd syringe was for in vitro study. Bone quality was confirmed by bone core biopsy performed 4 months post-operatively. This astonishingly rapid ossification was achieved allowing easy placement of 6 Nobel Replace RP implants.

Results: Show an adequately stimulated bone marrow can be a safe bioreactor enabling faster and better bone regeneration.

Conclusions: We present our way in obtaining a safe and highly useful autologous bioreactor using bone marrow stimulation, allowing generating high quantities of autologous stem cells committed for bone regeneration.

Conflict of interest: None declared.

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Jan 27, 2018 | Posted by in Oral and Maxillofacial Surgery | Comments Off on Activated autologous bone marrow for bone tissue engineering in the maxillofacial area: clinical application in preprosthetic implant surgery
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