The effect of a platelet-rich fibrin conduit on neurosensory recovery following inferior alveolar nerve lateralization: a preliminary clinical study

Abstract

This retrospective study aimed to assess the recovery of neurosensory dysfunction following modified inferior alveolar nerve (IAN) lateralization surgery compared to the conventional approach. Data from two groups of patients who underwent IAN lateralization in 2014 were included in this study. In one group, platelet-rich fibrin was placed over the IAN and this was protected with a collagen membrane conduit; the other group underwent the conventional IAN lateralization procedure. Implants were placed immediately. Neurosensory dysfunction was evaluated at 3, 6, and 12 months post-surgery. Demographic, neurosensory disturbance (NSD), subjective two-point discrimination test (TPD), and static light touch test (SLT) data were obtained. Twenty-three IAN lateralization procedures with the placement of 51 implants were performed in 14 patients. At the 6-month follow-up, the number of patients experiencing normal sensation was greater in the modified surgery group, but the 12-month follow-up results were the same in the two groups. More precise sensation was observed with the TPD in the modified group at 6 months, and the modified group demonstrated better SLT scores at 6 months. Although the two groups had comparable results at the 12-month follow-up, it was observed that the modified technique accelerated neural healing within 6 months and reduced the length of the discomfort period.

Severe resorption in the posterior mandible can occur as a result of tooth loss. When this happens, standard dental implants will encroach on the inferior alveolar nerve (IAN). There are several approaches for localized bone augmentation, namely guided bone regeneration, inlay and onlay grafts, bone tissue engineering, and nerve lateralization.

Nerve lateralization is a practical technique in posterior atrophic ridge augmentation of the mandible. In IAN lateralization, the nerve is freed from the IAN canal and is retracted laterally and placed on the lateral surface of the implants. A modification of this technique is IAN transposition, in which the IAN is cut anterior to the mental foramen and is freely displaced. However, these approaches are frequently accompanied by post-surgical neurosensory disturbance (NSD), with some studies estimating 30–75% nerve damage following surgery. This complication may overshadow the benefits of the surgery and this has led to debate over its application. As a result, several modifications have been made to IAN lateralization in order to decrease the post-surgical NSD, such as replacing the use of a bur with a piezoelectric surgery device for osteotomy, performing two osteotomies, and simultaneous bone expansion with expanders.

The positive effects of growth factors on accelerating nerve healing and regeneration has recently been reported in the literature. Platelet-rich fibrin (PRF) is composed of platelet concentrates on fibrin membranes. It possesses high regenerative potential due to the slow release of growth factors, such as transforming growth factor β1, platelet-derived growth factor β, and vascular endothelial growth factor. PRF has been used widely in oral and maxillofacial surgery and regeneration. A study on the healing potential of PRF on rat sciatic nerve defects demonstrated promising results, comparable in some aspects to those of the gold standard treatment – the autologous nerve graft.

Despite the vast literature on surgical approaches for minimizing post-surgical neural disturbance following IAN lateralization, no regenerative technique has yet been established for this purpose. The current retrospective cohort study aimed to assess the efficacy and post-surgical neural disturbances following a modified technique of IAN lateralization surgery.

Materials and methods

Patients

This retrospective cohort study evaluated the records of two groups of patients who had randomly received either conventional IAN lateralization surgery or the modified IAN lateralization surgery for implant treatment between January 2013 and January 2014. Eligibility for IAN lateralization surgery was confirmed by panoramic radiographs and cone beam computed tomography (CBCT) scans. The patients were eligible for enrolment if there was no systemic disease, no history of jaw trauma or oral surgery, and if there was less than 5 mm of bone height above the IAN to the bone crest. Patients were excluded if they had untreated periodontal disease, bisphosphonate use, knife edge alveolar ridges, parafunctional habits, unstable posterior occlusion, or signs of active or acute abscess. None of the patients were smokers. Written informed consent regarding the possibility of paresthesia following surgery was obtained from the patients. Due to the retrospective nature of this study, an exemption in writing was granted by the institutional review board. Demographic data, the surgical technique used, and postoperative follow-up results were extracted from the files. Patient records that lacked follow-up data or neurosensory assessments were excluded.

PRF preparation

Venous blood (20 ml) was obtained from the patient and placed in a sterile tube; this was centrifuged (EBA 20, Hettich, Tuttlingen, Germany) for 14 min at 2800 rpm (approximately 400 g ). Following centrifugation, there were three separate layers in the tube: cellular plasma at the top, a PRF clot in the middle, and red blood cells at the bottom. The PRF clot was separated and obtained using a sterile pincette.

Surgical procedure

Prior to surgery, the patients were given antibiotic prophylaxis comprising 2 g amoxicillin (Farabi Pharmaceutical Co., Isfahan, Iran) or 600 mg clindamycin in the case of a penicillin allergy. A non-steroidal anti-inflammatory agent (400 mg ibuprofen; Rouz Darou Pharmaceutical Co., Tehran, Iran) and a steroidal anti-inflammatory agent (5 mg oral dexamethasone; Iran Hormone Pharmaceutical Co., Tehran, Iran) were also given. All surgeries were performed under local anaesthesia by an experienced surgeon (A.K.).

The precise location of the mental foramen was determined using panoramic radiographs for all patients. A crestal incision was made distal to the canine tooth and extended to the anterior border of the mandibular ramus. Anterior releasing vertical incisions were made and a full thickness mucoperiosteal flap was elevated. An osteotomy was made from 3–4 mm distal to the mental foramen to 4–5 mm distal to the most distal planned implant position. The external outer cortical border of the mandible was removed either with a small round bur or as a thin block using a piezoelectric surgery device ( Fig. 1 ). In the case of a modification in the mental foramen area, a circular osteotomy was performed around the mental foramen with anterior extension beneath the canine tooth root. The incisive branch was also exposed and minimally displaced. Small round-edge smooth curettes were used to remove cancellous bone in order to gain access to the IAN. The neurovascular bundle was freed and moved laterally with a nerve hook and preserved by placing an elastic band (lacrimal probe; Katena Products, Inc., Denville, New Jersey, USA) below the nerve. During bone drilling and implant insertion, the nerve was retracted to reduce the risk of nerve damage ( Fig. 2 ).

Fig. 1
Thin box osteotomy design created with the piezoelectric surgery device. The bone block harvested with this method can be used as a barrier between the dental implant and the IAN or for possible simultaneous augmentation.

Fig. 2
Gentle retraction of the IAN during augmentation or the implant placement procedure (‘a’ indicates the IAN).

A collagen membrane (Jason membrane; Botiss Biomaterials GmbH, Berlin, Germany) was positioned beneath the nerve, the PRF was placed over the IAN, and then the collagen membrane was sutured with a resorbable suture (6–0 Vicryl; Ethicon Inc., Sint-Stevens-Woluwe, Belgium) ( Figs 3 and 4 ). After creating the conduit around the laterally displaced nerve, another suture was passed from the external surface of the collagen membrane and slung to the periosteum of the inferior border of the mandible ( Fig. 5 ). Harvested cortical bone or cancellous bone collected from the outer surface of the mandible was placed to cover the implant beneath the conduit. The overlying tissue was closed with a continuous horizontal suture (6–0 Vicryl; Ethicon Inc.) in a tension-free manner. The control group underwent the same procedure except that no PRF or collagen membrane was placed over the IAN.

Fig. 3
Schematic diagram of the surgical procedure: (A) a collagen membrane is passed beneath the IAN; (B) the PRF clot is placed over the IAN; (C) a conduit is made around the IAN and PRF with the collagen membrane.

Fig. 4
(A) Collagen membrane placed beneath the IAN. (B) PRF clot placed over the IAN. (C) Collagen membrane sutured with a resorbable suture to make a conduit around the IAN. (‘a’ indicates the IAN; ‘b’ indicates the collagen membrane; ‘c’ indicates the PRF; ‘d’ indicates the bone block placed for simultaneous augmentation.)

Fig. 5
Apical positioning of the collagen conduit by suturing of the membrane to the basal periosteum (‘a’), with simultaneous bone augmentation (‘b’).

Follow-up protocol

Patients were instructed to apply ice packs to the face for 2 h after surgery. They were prescribed amoxicillin 500 mg every 8 h for 7 days (or clindamycin 150 mg every 6 h for patients with a penicillin allergy) and analgesics (ibuprofen 400 mg for moderate pain or with acetaminophen codeine 300/10 mg for severe pain), and were given a single-dose (8 mg) dexamethasone intramuscular injection (Alborz Darou, Tehran, Iran). Furthermore, the patients were instructed to use 0.2% chlorhexidine mouthwash (Behsa Pharmaceutical Co., Tehran, Iran) two times a day for 1 week from the day after surgery. The patients were not allowed to wear a removable prosthesis. The sutures were removed at 10–14 days after surgery.

The patients were evaluated at 3, 6, and 12 months post-surgery. The tests used to assess the neural function of the lower lip and chin included an objective NSD test, subjective two-point discrimination (TPD) test, and static light touch (SLT) test. For the NSD evaluation, a questionnaire was used to determine whether the patient was experiencing numbness, tingling, or normal sensation in the area. The TPD test was performed by placing a sliding caliper on the skin; scoring was performed according to the distance sensed by the patient, as follows: <14 mm = score 2, 14–20 mm = score 1, >20 mm = score 0. The SLT test was carried out by applying pressure to the skin with a cotton-tipped applicator and the patients were asked to report any pressure sensation; scoring was as follows: >80% = score 2, <80% = score 1, no sensation = score 0.

Statistical analysis

The data analysis was performed using IBM SPSS 20.0.1 for Windows software (IBM Corp., Armonk, NY, USA). The mean and standard deviation values were calculated for continuous variables; discrete variables were expressed as frequencies.

Results

In this retrospective study, 23 nerve lateralization surgeries were performed in 14 patients aged between 44 and 62 years (mean age 53.93 years); five were males and nine were females. The surgery was performed bilaterally in nine patients and unilaterally in five patients. Seven patients underwent conventional IAN lateralization, while seven received the modified treatment. Fifty-one implants were inserted immediately following nerve lateralization. Neurosensory disturbance was evaluated at 3, 6, and 12 months and the TPD and SLT tests were performed at 6 and 12 months post-surgery.

At 3 months after surgery, all patients in both groups reported numbness, with the exception of one patient in the modified surgery group who reported normal sensation ( Table 1 ). At 6 months postoperative, three patients in the modified surgery group reported normal sensation compared to two patients in the conventional surgery group. At 12 months, the patients in both groups demonstrated a similar pattern of sensation; one patient in each group reported numbness, while the remaining patients had regained normal sensation.

Table 1
Neurosensory disturbance test results at the three follow-up sessions. a
Surgery group Sensation at 3 months Sensation at 6 months Sensation at 12 months
Normal Numbness Tingling Normal Numbness Tingling Normal Numbness Tingling
Conventional 0 7 0 2 3 2 6 1 0
Modified 1 6 0 3 3 1 6 1 0

The TPD test results at 6 months showed that all patients in the modified surgical technique group discriminated two points precisely (score 2), while in the conventional surgery group three patients scored 1 ( Table 2 ). The SLT test results at the 6-month follow-up demonstrated that two patients scored 2 in the modified surgery group, while two patients scored 0 in the conventional surgery group. At 12 months, all patients scored 2 in the SLT test, except for one patient in the conventional surgery group ( Table 3 ). Finally, patient satisfaction was compared between the two groups, and the modified surgery group expressed a higher level of satisfaction (100% vs. 71.4%).

Table 2
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Dec 15, 2017 | Posted by in Oral and Maxillofacial Surgery | Comments Off on The effect of a platelet-rich fibrin conduit on neurosensory recovery following inferior alveolar nerve lateralization: a preliminary clinical study
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