Helicobacter pylori is a microaerophilic bacteria, gram-negative, spiral shaped and mobile which is believed to be one of the main factors responsible for gastritis, gastric ulcers and gastric cancer. H pylori has direct cytotoxic properties that are harmful to the tissues, it was found that the bacterium causes the release of phospholipase A2, proteases and lipases. Chronic inflammation contributes eventually produced or may be causal of malignant changes in some cases.
Objetive: Find a direct relationship of Helicobacter pylori as a predisposing factor for oral cancer.
Materials and methods: We analyzed 40 patients, 2 samples each, a sample of saliva secretion and other dental plaque, 20 control (healthy patients) and 20 with diagnosed with oral cancer. Were taken aseptically, placed in a culture medium with bovine blood agar enriched with amphotericin B, vancomycin, cephalothin and trimethoprim. After 4 days of incubation, replanting is carried out and incubated for 4 days. Subsequently, DNA extraction takes place from 80 crops to be confirmed by real-time PCR, the presence of Helicobacter pylori.
Results: A total of 80 samples (40 of saliva and 40 dental plaque), H pylori was detected 13.75% (11samples). Of the 11positive samples were 63.63% of salivary secretion and 36.36% were from dental plaque. Note that both the saliva sample as dental plaque of 2 patients diagnosed with oral cancer floor of mouth were positive.
Conclusion: In our research we found no support for claiming that the presence of Hpylori could be considered as a predisposing factor in the presenceof oral cancer. However, we can deduce that Hpylori may be located more frequently in saliva and in patients with oral cancer floor of mouth.
Key words: Helicobacter pylori; oral cancer; saliva; dental plaque