Background and objectives: Platelet rich fibrin (PRF) is a second generation platelet concentrate widely used to accelerate soft and hard tissue healing and bone regeneration. This study investigated the effects of PRF application in early osseointegration in an animal model.

Material and methods: Twelve 4-month-old New Zealand white rabbits were used. in this experiment. After general anesthesia, 3–5 ml of blood was obtained from the central artery of the ear. It was then transferred to an eppendorf tube and centrifuged for 12 min at 2700 rpm and PRF was prepared. Four implant cavities were created in the right and left tibiae of the animals. Two of the sockets were selected randomly and PRF membranes were inserted (test group). No PRF application was made to the other sockets (control group). The rest of the PRF liquid was used to wash the implant that would be placed into the PRF-covered socket. Processing and staining of the bone samples obtained at 2, 3, and 4 weeks postsurgery were carried out using a standardized protocol and were stained with toluidine blue. The periimplant bone volume was evaluated histomorphometrically.

Results: The histomorphometric analysis revealed that the percentage of new bone formation and bone to implant contact ratio were significantly higher in the experimental group, especially in 3 and 4 weeks specimens ( P < 0.01).