Chapter 16 Vibrios, campylobacters and Wolinella Bacteria belonging to these three genera (together with others such as the genus Helicobacter) are morphologically similar, being Gram-negative curved bacilli. They are enteric pathogens of humans or part of the normal flora. Because of their unusual growth requirements (formate and fumarate needed), they have to be cultured in special media. Vibrios The genus Vibrio includes two important human pathogens, but their natural habitat is water. Vibrio cholerae causes cholera, while Vibrio parahaemolyticus causes a less severe diarrhoea. The main symptom of cholera is watery diarrhoea that can be fatal as a result of severe dehydration, water and electrolyte loss. Vibrio cholerae Habitat and transmission The habitat is water contaminated with faeces of patients or carriers; there is no animal reservoir. A life-threatening, watery diarrhoea (rice-water stools) is the characteristic disease. Characteristics Gram-negative slender bacilli, comma-shaped with pointed ends. Highly motile by means of a single polar flagellum. May be seen directly in stool samples by dark-field microscopy. Culture and identification Grows in alkaline conditions (pH 8.5–9.2 approximately): selective media for culture such as thiosulphate-citrate-bilesucrose (TCBS) medium are based on this property. This, together with biochemical tests and serology, helps identification. Serotyping is based on the somatic O antigens. All diarrhoea-producing strains of V. cholerae are designated as O1 and are subdivided into three major serotypes – the Ogawa, Inaba and El Tor strains. Pathogenicity V. cholerae has the ability to colonize the intestinal tract in very high numbers and about 108 cells per millilitre are seen in patients’ faeces. The cells attach to but do not invade the intestinal mucosa. Pathogenicity is due to secretion of an enterotoxin, which binds to ganglioside receptors on mucosal cells. After a lag period of 15–45 min, adenylate cyclase is activated and the cyclic adenosine monophosphate concentration inside the intestinal cells increases. This in turn leads to excretion of electrolytes and water and subsequent diarrhoea, leading to severe dehydration. Treatment and prevention Intravenous administration of fluids and electrolytes is essential for recovery. Oral administration of a solution containing glucose and electrolytes (oral rehydration therapy) is successful, but the patient must be capable of consuming the liquid by mouth. Severely ill patients are generally too weak to ingest fluids. Antibiotics (usually tetracycline) do not affect the disease outcome once the enterotoxin attaches to the intestinal cells, but they prevent subsequent attacks by reducing the number of toxin-producing V. cholerae cells in the intestine. Immunization with a whole-cell vaccine is of limited use. New vaccines are under development. Vibrio parahaemolyticus This vibrio requires a relatively high salt concentration for growth and is distributed worldwide in marine environments, for example, in South-East Asia. A common agent/> Only gold members can continue reading. Log In or Register to continue Share this:Click to share on Twitter (Opens in new window)Click to share on Facebook (Opens in new window) Related Related posts: 20: Chlamydiae, rickettsiae and mycoplasmas 27: Infections of the genitourinary tract 11: Streptococci, staphylococci and micrococci 32: Microbiology of dental caries 26: Infections of the gastrointestinal tract 4: Viruses and prions Stay updated, free dental videos. Join our Telegram channel Join Tags: Essential Microbiology for Dentistry 4e Jan 4, 2015 | Posted by mrzezo in General Dentistry | Comments Off on 16: Vibrios, campylobacters and Wolinella VIDEdental - Online dental courses
Chapter 16 Vibrios, campylobacters and Wolinella Bacteria belonging to these three genera (together with others such as the genus Helicobacter) are morphologically similar, being Gram-negative curved bacilli. They are enteric pathogens of humans or part of the normal flora. Because of their unusual growth requirements (formate and fumarate needed), they have to be cultured in special media. Vibrios The genus Vibrio includes two important human pathogens, but their natural habitat is water. Vibrio cholerae causes cholera, while Vibrio parahaemolyticus causes a less severe diarrhoea. The main symptom of cholera is watery diarrhoea that can be fatal as a result of severe dehydration, water and electrolyte loss. Vibrio cholerae Habitat and transmission The habitat is water contaminated with faeces of patients or carriers; there is no animal reservoir. A life-threatening, watery diarrhoea (rice-water stools) is the characteristic disease. Characteristics Gram-negative slender bacilli, comma-shaped with pointed ends. Highly motile by means of a single polar flagellum. May be seen directly in stool samples by dark-field microscopy. Culture and identification Grows in alkaline conditions (pH 8.5–9.2 approximately): selective media for culture such as thiosulphate-citrate-bilesucrose (TCBS) medium are based on this property. This, together with biochemical tests and serology, helps identification. Serotyping is based on the somatic O antigens. All diarrhoea-producing strains of V. cholerae are designated as O1 and are subdivided into three major serotypes – the Ogawa, Inaba and El Tor strains. Pathogenicity V. cholerae has the ability to colonize the intestinal tract in very high numbers and about 108 cells per millilitre are seen in patients’ faeces. The cells attach to but do not invade the intestinal mucosa. Pathogenicity is due to secretion of an enterotoxin, which binds to ganglioside receptors on mucosal cells. After a lag period of 15–45 min, adenylate cyclase is activated and the cyclic adenosine monophosphate concentration inside the intestinal cells increases. This in turn leads to excretion of electrolytes and water and subsequent diarrhoea, leading to severe dehydration. Treatment and prevention Intravenous administration of fluids and electrolytes is essential for recovery. Oral administration of a solution containing glucose and electrolytes (oral rehydration therapy) is successful, but the patient must be capable of consuming the liquid by mouth. Severely ill patients are generally too weak to ingest fluids. Antibiotics (usually tetracycline) do not affect the disease outcome once the enterotoxin attaches to the intestinal cells, but they prevent subsequent attacks by reducing the number of toxin-producing V. cholerae cells in the intestine. Immunization with a whole-cell vaccine is of limited use. New vaccines are under development. Vibrio parahaemolyticus This vibrio requires a relatively high salt concentration for growth and is distributed worldwide in marine environments, for example, in South-East Asia. A common agent/> Only gold members can continue reading. Log In or Register to continue Share this:Click to share on Twitter (Opens in new window)Click to share on Facebook (Opens in new window) Related Related posts: 20: Chlamydiae, rickettsiae and mycoplasmas 27: Infections of the genitourinary tract 11: Streptococci, staphylococci and micrococci 32: Microbiology of dental caries 26: Infections of the gastrointestinal tract 4: Viruses and prions Stay updated, free dental videos. Join our Telegram channel Join