Stability of caries-affected and sound dentin treated with collagen cross-linkers

Objectives . To evaluate the effect of various collagen cross-linking agents on the thermal stability of caries-affected and sound demineralized dentin using a differential scanning calorimetry (DSC) assay.

Materials and methods : Five sound and ten carious human molars were selected. Specimens were cut into 0.2 mm thick slabs from mid-coronal dentin and trimmed to a final dimension of 2.0 × 2.0 × 0.2 mm. To determine caries-affected dentin, sections were stained with a caries detector and sectioned in the same manner described above. Specimens were fully demineralized and randomly divided into 5 experimental groups and a control (no treatment). The cross-linkers used were: 2 synthetic [glutaraldehyde: GD, carbodiimide: EDC] or 3 naturally occuring [grape seed extract: GSE, tannic acid: TA and cocoa extract: COA]. Agents were prepared in distilled water and pH adjusted to 7.2. Samples were treated for 60 min, thoroughly rinsed, sealed in high pressure stainless steel pans, weighted and temperature denaturation ( T d ) was assessed using DSC (Pyrus 1 PerkinElmer) scanned from 25 °C to 130 °C at a rate of 5 °C/min. Cooling and reheating confirmed that the collagen denaturation was irreversible.

Results : The data is depicted in Table [mean (standard deviation)]. There was no significant interaction between factors (substrate vs. treatment, p = 0.990). No statistically significant difference was observed between the T d values of caries-affected and sound dentin ( p = 0.228). The application of collagen cross-linkers resulted in statistically significant increase on T d values ( p = 0.0001). No differences were observed between collagen cross-linkers ( p > 0.05). Multiple peaks up to 106.80°C were observed for GSE, TA and COA dentin treated matrix.

Nov 30, 2017 | Posted by in Dental Materials | Comments Off on Stability of caries-affected and sound dentin treated with collagen cross-linkers
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