Objectives . The purpose of this study was to evaluate, by means of tensile bond strength (TBS) test, the influence of Mefp-1-based peptides chain configuration as well as DOPA aminoacid role on chemical interaction to zirconium oxide ceramics.

Materials and methods . Aminoacid sequences derived from Mytilus edulis foot protein 1 (Mefp-1) were synthesized applying the Fmoc-strategy (A433 Solid Phase Peptide Synthesizer, Applied Biosystems). The purification was accomplished by HPLC (BioCad 700E; PerSeptive BioSystems). The original sequence was diversified into six experimental peptides (see table of results), being four of them single deca-peptides and two duplicated deca-peptides. The role of DOPA adhesion was evaluated by testing it alone, swapping its position in the peptide sequence and doubling its concentration in the sequence. Onto the surface of 3 × 3 × 9 mm zirconium oxide ceramic bars, 2.5 μl of peptide solution in 0.1% TFA was applied and oxidized with 1 μl saturated KIO ₃ . The zirconia bars were bonded together by one surface in a perpendicular orientation (cross-shape) and stored for 7 days under a 5-g brass weight. The bonded specimens were mounted in an universal testing machine (Zwick/Roell) and pulled in tension by a wire. Tensile bond strength was given in MPa by dividing the force in N by the adhesion area (≅9 mm ² ). Data were analysed by one-way ANOVA and mod. LSD-test ( p < 0.05).

Results .

Sequence	TBS (MPa) in zirconia
DOPA	1.0 ± 0.65 (d)
Ala-Lys-Pro-Ser-Tyr-Hyp-Hyp-Thr- Tyr -Lys	2.71 ± 1.47 (a,b)
Ala-Lys-Pro-Ser-Tyr-Hyp-Hyp-Thr- DOPA -Lys	1.74 ± 1.25 (b,c,d)
Ala-Lys-Pro-Ser- DOPA -Hyp-Hyp-Thr- Tyr -Lys	1.49 ± 1.18 (c,d)
Ala-Lys-Pro-Ser- DOPA -Hyp-Hyp-Thr- DOPA -Lys	2.41 ± 1.45 (a,b,c)
Ala-Lys-Pro-Ser-Tyr-Hyp-Hyp-Thr-Tyr-Lys-Ala-Lys-Pro-Ser-Tyr-Hyp-Hyp-Thr- DOPA -Lys	3.40 ± 1.29 (a)
Ala-Lys-Pro-Ser-Tyr-Hyp-Hyp-Thr- DOPA -Lys-Ala-Lys-Pro-Ser-Tyr-Hyp-Hyp-Thr- DOPA -Lys	2.61 ± 1.31 (a,b,c)

Only gold members can continue reading. Log In or Register to continue

Share this:

• Click to share on Twitter (Opens in new window)
• Click to share on Facebook (Opens in new window)

Related

Related posts:

1. Abstracts from the Academy of Dental Materials conference in Portland, October 2009. Poster Index by presenter
2. Effect of instrument compliance on polymerization shrinkage stress measurements of composites
3. Testing bond strength: A review of the literature
4. Compatibility of self-etching dentin adhesives with resin luting cements
5. Effect of particle deposition protocols on flexural strength of zirconia after cyclic loading
6. A novel experimental method for the local mechanical testing of human coronal dentin

Stay updated, free dental videos. Join our Telegram channel

Join