Long-term follow-up of autogenous canine transplants with application of guided bone regeneration


The aim of this study was to evaluate the aesthetics and outcome of autotransplantation of impacted canines unsuitable for orthodontic realignment using a combination of surgery with guided bone regeneration and orthodontic treatment. Ten severely impacted canines in nine consecutive patients (mean age 14.8 years) were included. The recipient mesiodistal space was created orthodontically and the recipient socket prepared using dental implant drills. Following transplantation, bone defects were grafted using guided bone regeneration, teeth were aligned and occlusions adjusted orthodontically. Patients were followed-up every 6 months for an average of 7.1 years (range 2–11 years). The mean pink aesthetic score was 13.33 ± 0.87. Pocket depths were less than 3 mm. Radiographic examination showed an uninterrupted periodontal space and lamina dura in seven cases, unclear periodontal space in two, and replacement resorption in one case. The analysis showed that canine transplantation combined with guided bone regeneration and orthodontic treatment gives acceptable and predictable aesthetic results.

Impacted teeth are those with a delayed eruption time or that are not expected to erupt completely based on clinical and radiographic assessment. Studies have reported that the incidence of tooth impaction varies from 5.6% to 18.8% of the population. The maxillary canine is an important tooth within the dentition, having a role in aesthetics, lip support, and masticatory efficiency. The permanent maxillary canine is the second most frequently impacted tooth in the dental arch after the third molar, and the prevalence of its impaction is 1–2% in the general population. Eighty-five percent of impacted maxillary permanent canines are palatal, and 15% are labial impactions. The incidence of impacted mandibular canines is 0.35%.

Treatment options for impacted teeth include: (1) surgical exposure with orthodontic alignment; (2) extraction of the impacted tooth with simultaneous orthodontic alignment in circumstances where arches are crowded; (3) extraction of the impacted tooth, followed by later implant placement when the jaw bone is fully grown, usually after 18 years of age. In cases where an impacted tooth is severely displaced or the impacted position is too deep, orthodontic alignment is impossible, and autotransplantation can be considered as a treatment option. Compared with an implant, transplanted teeth can erupt in harmony with the adjacent teeth when the jaw bone is not fully grown. The healing of transplants is rapid and they have acceptable function almost immediately. Moreover, transplants have good aesthetic results, as their natural emergence profile and the natural appearance of enamel and the crown form are maintained. The total cost of transplantation is also much lower than implant treatment.

The frequent occurrence of bone defects in the original position of the impacted canine after its extraction is an issue, and complications that are associated with the surgical removal of impacted teeth include periodontally compromised adjacent teeth and osseous defects. When donor teeth are placed into the recipient site with poor buccolingual spacing, roots can protrude through a bone dehiscence. Graft materials should therefore be placed over the exposed root in order to create space for bone regeneration. Penarrocha et al. suggested that after removing the ectopically positioned canines, implants could be placed and anchored bicortically in the sockets and floor of the nasal fossae and maxillary sinus, and the bone defects remaining around the implants could be filled with collected allograft shavings. In the treatment of bone defects around an implant, Simion et al. used polylactic acid/polyglycolic acid membranes in association with autogenous bone chips as a space-maker and stabilized with fixation screws or nails. Bone substitutes and guided bone regeneration (GBR) have also been used extensively in areas of deficient bone volume as spacers under membranes. However, very few clinical studies have investigated the application of GBR in canine autotransplantation when osseous defects are present after the removal of impacted teeth.

The purpose of this study was to investigate the indications for canine transplantation with a combination of GBR techniques and orthodontic treatment. The indications for this treatment option and surgical techniques that affect the prognosis were investigated over a long follow-up period. Graft survival and functional success rates were assessed in addition to aesthetic outcomes.

Materials and methods

The institutional ethics committee approved the research protocol.

Clinical records

Over a 9-year period, from 2001 to 2009, nine consecutive patients (five female, four male; 10 transplanted teeth) were referred for dental autotransplantation. Two of the teeth were labial impacted maxillary canines, three were palatal impactions, three were mandibular impacted teeth, and two were labial impacted canines where orthodontic traction had failed. The average age of the patients at the time of transplantation was 14.8 years (range 13–17 years).

Preoperative orthodontic treatment

The mesiodistal space of the recipient site, equivalent to the mesiodistal diameter of the contralateral canine, was created with preoperative orthodontic treatment. Malocclusion, especially where the opposing teeth were overgrown, was corrected to prevent the vertical position of the transplant being affected and the profile of the transplant being badly impaired by occlusal adjustment and occlusal trauma ( Figs 1 and 2 ).

Fig. 1
Panoramic radiograph at the initial examination reveals an ectopically erupted mandibular canine.


Fig. 2
The mesiodistal space of the recipient site was created with preoperative orthodontic treatment.

Surgical procedure

Preparation of the donated tooth

Local anaesthetic (lidocaine 2% with epinephrine 1:100,000) was administered to the patient. An incision was made and a full-thickness flap reflection was performed in order to fully expose the surgical site. An ostectomy was performed to remove the donated tooth atraumatically, and care was taken to preserve as much periodontal ligament (PDL) on the root as possible. After the diameter and length of the root were measured, the donated tooth was replaced in the extraction socket for preservation ( Fig. 3 ). The extraoral transplantation time should be less than 30 min.

Fig. 3
The donated tooth was removed atraumatically.

Surgical treatment of the recipient area

The recipient bed was prepared a little larger than the donor using dental implant drills (NobelReplace Dental Implant, Nobel Biocare AB, Sweden) of increasing diameter, under irrigation with abundant sterile saline solution in order to avoid thermal damage to the bone ( Fig. 4 ). The donated tooth was transplanted from the original area to the recipient bed as soon as possible ( Fig. 5 ). The average time between the removal of the impacted tooth and its placement in the new socket was 15 min. In circumstances where a retained deciduous tooth is combined with deficient bone volume, labial dehiscence and bony defects may appear when the recipient bed is prepared. In such cases, bone defects were grafted after transplantation using a GBR technique. Bio-Oss and Bio-Oss Collagen (Geistlich Pharma AG, Wolhusen, Switzerland) and a few autogenous bone chips collected during the ostectomy were used to fill the labial sides of transplants with insufficient bone volume, and resorbable membranes (Bio-Gide) were then applied to cover the defects ( Figs 6 and 7 ).

Fig. 4
The recipient bed was prepared with dental implant surgery.


Fig. 5
A clinical view of the transplantation of the canine to its normal position.


Fig. 6
The use of Bio-Oss and Bio-Oss Collagen in a largely osseous defect.


Fig. 7
The application of resorbable membranes (Bio-Gide) to cover the defect.

Fixation of the transplant

The transplanted tooth was stabilized with non-absorbable surgical sutures, or was tied to the adjacent tooth with a ligature by bonding an orthodontic bracket directly onto the tooth surface. Sutures and ligatures were removed 2–3 weeks after the operation to avoid ankylosis ( Fig. 8 ).

Fig. 8
The transplanted tooth could be stabilized by surgical suturing or could be tied to the adjacent tooth using orthodontic brackets and a ligature.

The dental surgeon ensured that 40 μm articulating paper could pass between the teeth without resistance, ensuring that the transplanted tooth was placed out of occlusion. The configuration of the transplanted tooth was adjusted in accordance with the contralateral teeth. Patients received postoperative antibiotics during the first week and teeth were rinsed with 0.12% chlorhexidine for a further 2 weeks.

Clinical observations

The transplanted teeth were assessed at 2, 4, 6, 8, 10, and 12 weeks after placement and then reevaluated every 6 months. The following information was recorded: the pink aesthetic score (PES), tooth mobility, and the probing pocket depth (PPD). Postoperative peri-apical radiographs were taken to monitor root resorption, periodontal condition, development of the lamina dura, and ankylosis every month postoperatively until the sixth month, and then once every year. After the initial 6 weeks, vitality testing was performed with a pulp tester (Parkell, Farmingdale, NY, USA). At the 6-month postoperative assessment, transplanted teeth that were found to react negatively to pulp testing were treated endodontically with calcium hydroxide. The final endodontic treatment was performed within a year post-transplantation.

Radiographic examination

Panoramic radiography and cone beam computed tomography (CBCT) was used prior to transplantation to confirm the exact position of the impacted canine and to design the operative pathway. Postoperative peri-apical radiographs were taken to monitor root resorption, periodontal condition, the development of lamina dura, and ankylosis ( Figs 1, 9 and 10 ).

Fig. 9
Postoperative panoramic radiograph.


Jan 21, 2018 | Posted by in Oral and Maxillofacial Surgery | Comments Off on Long-term follow-up of autogenous canine transplants with application of guided bone regeneration
Premium Wordpress Themes by UFO Themes