Background and objectives: Determine the effect of biocoating with protein (A) on discs and biocoating stability on the implant after insertion in animal bone.

Methods: Four MIS-implants (3.75 mm × 10 mm) and four MIS-discs (14.0 mm × 2.0 mm) were used. Discs were divided in two groups: AB/AE and PLATES, one discs per group was biocoated with Protein A, the other one was control. The four implants were divided: Two implants (1 and 3) were treated with protein A, and two (2 and 4) were no treated. 3 and 4 were introduced to animal bone. Disc and implant treatments were for 24 h in an incubator at 37 °C. Discs and implants were analyzed by AFM.

Results: Discs: AB/AE without surface treatment showed higher roughness than PLATES. AB/AE with Protein A reduced roughness compared with control. PLATES increased their roughness. Implants: sample 4 looked much rougher than sample 3, but the roughness decreased compared with sample 2.

Conclusions: It is possible to add proteins to Ti surface. Biocoating with Protein A is effective. Submersing discs and implants is an effective method for biocoating. AB/AE implant titanium surfaces biocoated with Protein A are stable. AFM is an effective instrument for detecting biocoating in Ti surface.