Background and aim: Ameloblastic carcinoma is a rare malignant epithelial odontogenic tumour that histologically retains the features of ameloblastic differentiation and exhibits cytological features of malignancy in the primary or recurrent tumour. It may develop within a preexisting ameloblastoma or arise de novo or from an odontogenic cyst. Epidemiological evidence shows that human cancer is generally caused due to genotoxic factors. Genes involved in the susceptibility of cancer, including genes involved in metabolism or detoxification genotoxic environment and genes are controlling DNA replication. Nowadays, gene polymorphism has an important role in development of malignant tumour.

Materials and method: We investigated a case series study of ameloblastic carcinoma and ameloblastoma by clinical and pathological findings. In these cases we also investigated the H391Y polymorphism in PKM2 and the c.196C> polymorphism in MAPK8IP2 compare with positive samples to show the role of PKM2 and MAPK8IP2 polymorphism in these tumours. The DNA was extracted separately from specimens in paraffin sections of the tumour. Polymorphism of these genes was determined by RFLP-PCR (polymerase chain reaction-restriction fragment length polymorphism) method.

Results: The H391Y polymorphism in PKM2 and the c.196C> polymorphism in MAPK8IP2 were not recognized in all of the sample cases.

Conclusion: We didn’t find H391Y polymorphism in PKM2 and the c.196C> polymorphism in our samples. Matter of our population may be the fact of different result between our investigation and other investigation. The techniques and the size of samples may be changed to improve these investigations. We suggest sequencing of those genes to find any SNPs or other changes in the genes. The RFLP-PCR method has been used in this study and may nucleotide changes in the regions of the gene, outside the effect region of enzymes used in this study, there is one and therefore requires sequencing method is examined next.