Comparative study of alternative MSC sources for bone regeneration over a ceramic foamed support

Introduction: One of the newly developed ideas to build ceramic scaffolds, of relative simplicity, is based on polyurethane foams. On the other hand, one of the most recently explored sources of MSC is that of dental pulp.

Objective: Enhance the ceramic scaffold fabrication technique based on polyurethane foams and compare the development on them of MSC from different sources.

Methodology: A mixture of tri-calcium phosphate powder using a specific binder was sintered employing the embedded polyurethane foam technique, obtaining a microporous scaffold. On the other hand, MSC from adipose, umbilical cord and dental pulp sources were isolated, multiplied and differentiated. The latter source of MSC was obtained from embrionary dental pulp tissue from third molars extracted during the formation period.

Results: Sintered ceramic scaffold favored the cellular growth over a microporous surface. A comparative analysis demonstrated differences in the early and late expression markers for bone tissue, in accordance to the source from where the MSC came from and the time of observation. Larger indices of mineralization and thus expression markers were obtained for osteocalcin in MSC from dental pulp.

Discussion: A ceramic scaffold fabricated from polyurethane foam in conjunction with MSC from embrionary pulp appears to be a good alternative for the bone tissue regeneration. Nonetheless, it is still required to characterize more specifically the cellular nature of the cellular differentiation that it is obtained from this MSC source (bone v/s dentin).

Conflict of interest: None declared.

Only gold members can continue reading. Log In or Register to continue

Feb 5, 2018 | Posted by in Oral and Maxillofacial Surgery | Comments Off on Comparative study of alternative MSC sources for bone regeneration over a ceramic foamed support
Premium Wordpress Themes by UFO Themes